recombinant tnf alpha
I would like to ask you for help. TNF-α plays an important role in the immune response to microbial invasions and in the necrosis of specific tumors.
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98 by HPLC analyses.
. Human Recombinant TNF-alpha Overview. See Related Products For research purposes only not for human use Applications Our bioassay Alomone Labs Recombinant human TNF-alpha protein induces cytolysis in HeLa cells. We currently have two TNFalpha ELISA kits specific for mouse.
Find supporting information and directions for use in the Product Information Sheet or explore. The identity of the protein was confirmed by MALDI-TOF mass spectrometry. The ED 50 as determined by the dose-dependant Killingapoptosis of L-929 cells is 071ngmL corresponding to a Specific Activity of 141 x 10 6 IUmg.
Showing 1 - 4 of 4 FAQ. The plate was incubated for 23 hours at 37C and then. To study the effect of human recombinant tumour necrosis factor alpha TNF alpha on contracture development and changes in the ionic composition of the external bathing solution of electrically stimulated rat hemidiaphragm preparations in vitro.
It is expressed as a 26 kDa membrane-bound protein and free soluble 17 kDa monomer which forms homotrimers in circulation. Recombinant Human TNF-α is a soluble 157 amino acid protein 174 kDa which corresponds to C-terminal extracellular domain of the full length transmembrane protein. Molecular Mass The 157 amino acid recombinant protein has a predicted molecular mass of 17352 Da.
A The biological activity of Mouse Recombinant TNF-alpha was tested by its ability to induce cytolysis of L929 cells growing in the presence of actinomycin D. Products Building Blocks Explorer Technical Documents Site Content Papers Genes. FAQs for TNF-alpha Recombinant Protein NBP2-35185.
High Purity 98 HPLC. Description Product name Recombinant human TNF alpha protein Active See all TNF alpha proteins and peptides Biological activity Fully biologically active when compared to standard. Cell viability was measured after 19 hours of culture using a fluorometric assay method.
Recombinant Human tumor necrosis factor alpha is an E. Tumor necrosis factor-α TNF-α is a pro-inflammatory cytokine that activates NF-kB MAPK and PI3KAKT. The TNF-alpha is purified by standard chromatographic techniques.
The protein was obtained from the last chromatography step as a trimer while purity was at least 96 as estimated by SDS-PAGE. Find recombinant tnf-alpha and related products for scientific research at MilliporeSigma. Western blot analysis was performed using conditioned media from HUVEC cells treated with 10 ngmL of human TNF-alpha recombinant protein Product PHC3013 for 12 hours.
Mouse TNF-alpha Recombinant Protein Product RMTNFAI was serially diluted 14 in triplicate starting from a concentration of 100 ngmL in assay media containing 1 µgmL Actinomycin D 2X Gentamicin and 10 Horse Serum and 100 µL was added to each well. Tumor Necrosis Factor TNFSF2 Cachectin Differentiation-inducing factor DIF Necrosin Cytotoxin. Ad Cell culture proteins providing consistency with market leading QC utilising HPLC and MS.
Recombinant Human TNF-alpha Protein Val77-Leu233 HPLC-verified 10602-HNAE is expressed in E. With high purity high biological activity high stability and other superior features you can use this Human TNF-alpha protein for relevant bioassay and related research. Tumore necrosis factor-α TNF-α also known as cachectin is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme TACE to release the soluble 17 kDa monomer which forms homotrimers in circulation.
TNF as also known as TNF-alpha or cachectin is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor TNF superfamily. ISO9001 manufactured ensuring highest purity ultra-low endotoxin is consistently achieved. Catalog numbers KA0257 and NBP1-92670.
Coli -derived recombinant protein corresponding to the C-terminal domain of human tumour necrosis factor alpha TNFa a pro-inflammatory cytokine with cytotoxic effects on various tumour cells. Recombinant mature TNF-alpha was correctly folded as assessed by CD spectroscopy and its biological activity was confirmed by an L929 cell assay. Recombinant human TNF alpha protein See all TNF alpha proteins and peptides Biological activity The ED 50 as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D is 005 ngmL corresponding to a specific activity of 2 x 10 7 unitsmg.
High Purity 98 HPLC. Anti-CX3CL1 Fractalkine polyclonal antibody eBioscience Product 14-7986-81 was used to probe the blot and an 85 kDa band corresponding to the soluble cleaved form of CX3CL1 was observed. Human TNF-α amino acids Val77-Leu233 Accession NM_000594 was expressed in E.
Purity 98 SDS-PAGE. Recombinant Human Tumor Necrosis Factor alpha with HSA Tumor necrosis factor-alpha TNF-α is a pleiotropic inflammatory cytokine produced by several types of cells predominantly activated macrophages. Tumor Necrosis Factor-a Human Recombinant produced in EColi is a single non-glycosylated polypeptide chain containing 158 amino acids 157 aa.
Ad Cell culture proteins providing consistency with market leading QC utilising HPLC and MS. Ad Expert Technical Support. Human recombinant TNF alpha affects rat diaphragm muscle in vitro Abstract Objectives.
Product Details Target Species Human Product Form Purified recombinant cytokine - lyophilized. ISO9001 manufactured ensuring highest purity ultra-low endotoxin is consistently achieved. Among these TNF-alpha proteins there are 4 Human TNF-alpha protein 2 Mouse TNF-alpha protein 1 Ferret TNF-alpha protein 1 Canine TNF-alpha protein 1 Rat TNF-alpha protein 1 Cynomolgus Rhesus TNF-alpha protein 1 Cynomolgus TNF-alpha protein.
There are 11 TNF-alpha protein produced in house with high quality which are covering various species. Recombinant human TNF-alpha protein T-100 is a highly pure recombinant and biologically active protein. Applications Products Services Support.
Ad Expert Technical Support. I need to know about TNF-alpha antibody kits for mice. The DTT-reduced protein and non-reduced protein migrate at approximately 16kDa by SDS-PAGE.
I need an antibody for Elisa to detect human TNF alpha in a supernatant from. The N-terminal amino acid is Val. Of the mature human TNF-alpha and an N-terminal methionine and having a molecular mass of 175kDa.
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